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. 2011 Sep 30;12(11):1160–1166. doi: 10.1038/embor.2011.184

Figure 2.

Figure 2

Spindlin1 is an H3K4me3-binding protein. (A) Nucleosome pulldown experiments showed that recombinant Spindlin1 recognized nucleosomes containing H3Kc4me2 and H3Kc4me3. (B) Isothermal titration calorimetry analysis showed binding between Spindlin1 and H3K4me3 peptide with a Kd of about 0.8 × 10−6 mol/l, binding between Spindlin1 and H3K4me2 peptide with a Kd of about 6 × 10−6 mol/l, and binding between Spindlin1 and H3K4me1 peptide with a Kd of about 11 × 10−6 mol/l. (C) A modelled structure of Spindlin1 and H3K4me3 complex, showing key residues caging the trimethylated H3K4. (D) Spindlin1 Y170A and F141A mutants showed reduced binding to mononucleosomes containing H3Kc4me3. (E) Spindlin1 Y170A and F141A mutants showed impaired interaction with H3K4me3 peptide.