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. 2011 Sep 30;12(11):1160–1166. doi: 10.1038/embor.2011.184

Figure 5.

Figure 5

Spindlin1 facilitates the expression of ribosomal RNA (rRNA) genes. (A) Reverse transcription–polymerase chain reaction (RT–PCR) results revealed elevated pre-rRNA levels upon Flag–Spindlin1 induction. (B) RT–PCR results showing impaired stimulation of pre-rRNA expression in cells expressing the Spindlin1 mutants (left panel); the expression levels of the Flag–Spindlin1 were also determined by RT–PCR (right panel). (C) Western blot analyses (left panel) and RT–PCR (right panel) verified the efficiency of Spindlin1 knockdown in HeLa cells. (D) RT–PCR results showed significantly reduced pre-rRNA levels on Spindlin1 knockdown. Error bars represent the standard deviation calculated from three independent experiment repeats. ***P<0.002; **P<0.01; *P<0.05; and #P>0.1. mRNA, messenger RNA; siRNA, small interfering RNA; β-Gal, β-galactosidase.