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Claudin-1 is not expressed in parenchymal CNS microvessels. mRNA expression level of Claudin-1 and Claudin-5 in parenchymal CNS microvascular endothelial cells was tested by quantitative RT-PCR with the s16 ribosomal protein (Rps16) set to 1.0. As a source for RNA mouse brain microvessels were used immediately after their isolation. Data express mean values of 5 qPCRs carried out in triplicates ± standard error. b.d. below limit of detection
