Fig. 4.

TET-regulated claudin-1 does not influence the number of inflammatory cuffs in the brain. Inflammatory cuffs were counted in a total of 10 double transgenic (dtg) and 15 single transgenic (stg) Tie2-tTA//TRE-claudin-1 mice of lines 23974 and 23949 in 168 versus 147 brain sections, respectively. A total of 1020 versus 1338 parenchymal cuffs (P), 474 versus 510 leptomeningeal cuffs (M) and 197 versus 170 periventricular cuffs (V) in double transgenic versus single transgenic mice, respectively, are included into the analysis. Each dot represents the mean number of inflammatory cuffs/per brain section of one mouse. Horizontal lines depict median for each group. Mann–Whitney U analysis revealed no significant differences comparing the number of cuffs in stg versus dtg mice