Fig. 5.

Notch 1 expression in neural progenitor cells. a The treated neural progenitor cells (NPCs) with sNgR-Fc (500 ng/ml), MAG (myelin-associated glycoprotein) or MW167 (a Notch1 inhibitor) were used for Notch1 activation using Western blotting. b Quantitative analysis of Western blotting. The levels of NICD (Notch intracellular domain) and Hes 5 were increased with the different dosages of sNgR-Fc, but were reversed by the administration of MAG and MW167. *P < 0.05, **P < 0.01 compared to the control group, MW167 and MAG-Fc groups. ^# P < 0.05, ^## P < 0.01 compared to the control group. The experiments were repeated 3 time. c–f Coexpression of Notch 1 (green) with Nestin (red) in the cultured NPCs after sNgR-Fc treatment (500 ng/ml). The nucleus was counterstained with DAPI (4′,6-diamidino-2-phenylindole)