Figure 3.

In vitro translated γ- and δ-sarcoglycan interaction with a GST/FLN2 fusion protein. (A) ³⁵S-labeled in vitro translated products were mixed with a GST/FLN2 fusion protein containing FLN2 amino acids 2,128–2,688. Lanes 1 and 2 contain translated δ-sarcoglycan protein mixed with fusion protein (lane 1) or GST protein alone (lane 2). Lanes 3 and 4 contain translated γ-sarcoglycan mixed with fusion protein (lane 3) or GST protein alone (lane 4). Lanes 5–8 contain either the carboxyl terminus of dystrophin (lanes 5 and 6) or syntrophin (lanes 7 and 8) combined with FLN2 fusion protein (lanes 5 and 7) or GST protein alone (lanes 6 and 8). (B) Supernatants of the above reactions corresponding to the respective lanes in A (lanes 1–8), showing that translated product was present in all tubes.