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. 2000 Jan 10;148(1):73–86. doi: 10.1083/jcb.148.1.73

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Partial, but not complete, inactivation of lin-5 delays exit from mitosis, consistent with a lin-5 function in both mitotic arrest and chromosome segregation. Arrested early L1 animals were shifted from 15 to 23°C (left) or 25°C (right), stimulated to develop synchronously by addition of food, and examined using Nomarski optics. The time in mitosis, determined as the time from disappearance of the nucleolus until reformation of the nuclear envelope, was recorded for the first (abortive) divisions in the P cell lineages. L1 larvae scored were of wild-type (WT), lin-5(e1348)/+, lin-5(e1348), or lin-5(ev571ts) genotype.