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. 2000 Jan 10;148(1):87–100. doi: 10.1083/jcb.148.1.87

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Confocal analysis of cross-linking proteins in developing bristles. A macrochaete from a 36-h pupae was imaged after staining with rhodamine-phalloidin to illustrate F-actin (a), and with an antibody against the forked proteins to show the forked protein distribution (b). Of interest is that except for a diffuse staining at the tip of the bristle, the phalloidin staining is confined to the actin bundles. The forked proteins are present in large amounts at the tip of the bristle (arrow). Along the rest of the bristle shaft, the forked proteins are confined to the actin bundles. A growing macrochaete was imaged after rhodamine-phalloidin staining (c) and staining with antibodies against fascin (d). Of interest is that the fascin which seems to be present in large amounts is present throughout the bristle cytoplasm, not confined to or concentrated in the actin bundles. A mature macrochaete (48 h) was imaged after rhodamine-phalloidin staining (e) and after staining with antibody against fascin (f). Here fascin is localized primarily to mature actin bundles. In addition, some cytoplasmic fascin is still apparent. Bars, 5 μm.