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Effect of the KcsA cytoplasmic domain. Single-channel currents were recorded in a symmetrical solution containing 200 mM KCl. (A) Schematic representation of KcsA, MthK TM, and MthK TM–KcsA cyto channels. (B) Representative current records of each at pH 4 and pH 7 at 40 mV. (C) Open probabilities (ordinate) at 40 mV versus pH (abscissa; n = 4–14).
