Figure 4.

Effects of E146 and D149 on gating. Six mutants with different mutations in the cytoplasmic domain had their activities measured. (A) Amino acid sequences of the cytoplasmic domain for KcsA and the mutants. Shaded letters indicate differences from the KcsA sequence. (B) Open probabilities at pH 7 and 40 mV (n = 4–14). (C) Representative current records of mutant 5 at pH 4, 7, and 9, and 40 mV.