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Ruggedness testing
| Parameter | Conditions |
|---|---|
| Ion-pairing concentration, mM | 5, 10, 20, 30 |
| Organic modifier concentration, % | 10, 20, 30 |
| Mobile phase pH | 4.0, 5.0, 6.0, 7.0, 8.0, 8.2 |
| Column temperature, °C | 25, 30, 35 |
| Ion-pairing agents | Hexanesulfonic acid, dodecylsulfonic acid |
