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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Nov 4;31(2):368–375. doi: 10.1161/ATVBAHA.110.218149

Figure 6. Downregulation of myocardin and miR-1 in neointima lesions in mouse carotid arteries.

Figure 6

Neointima lesions were created by ligation of carotid arteries of mice for 15 d as described in Materials and Methods. A and B: Micrographs of arterial cross-sections with H & E staining with (B, injured) or without (A, control) ligation. M, media; NI, neointima; L, lumen. C–E: Control and injured arterial segments were harvested for extraction of miRNA or mRNA, followed by measuring the expression of miR-1 (C), myocardin (D) or Pim-1 (E) using PCR. The expression levels of miR-1 and myocardin or Pim-1 were normalized by U6B and GAPDH, respectively. * indicates significant difference compared with control group (p < 0.001, n=7 for miR-1; p < 0.05, n=5 for myocardin and Pim-1).