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. Author manuscript; available in PMC: 2012 Apr 5.
Published in final edited form as: Cold Spring Harb Symp Quant Biol. 2011 Apr 5;75:395–401. doi: 10.1101/sqb.2010.75.040

Figure 4.

Figure 4

DASH/Dam1 complex: formation of rings on microtubules (MTs). (A) Image of negatively stained MT “decorated” with DASH at high concentration. (B) Image of negatively stained MT decorated with DASH at lower concentration, followed by addition of gold-tagged antibody (against the His tag on the DASH components). The antibody clusters the rings in pairs; with no antibody added, the rings are randomly spaced (not shown). (C,D) Diagrams showing flexible connections between a DASH ring and an MT. The connections can adapt to different registers of the MT protofilaments. (A and B, Reprinted from Miranda et al. 2005 with permission from Nature Publishing Group © 2005; C and D, reprinted from Miranda et al. 2007 with permission from American Society for Cell Biology © 2007.)