Skip to main content
. 2011 Nov;18(11):693–702. doi: 10.1101/lm.2273111

Figure 2.

Figure 2.

BLA/DH disconnection following cocaine memory reactivation attenuates subsequent drug context-induced cocaine-seeking behavior relative to VEH or ipsilateral ANI + B/M treatment. (A) Schematic depicting the timeline for Experiment 1. Cocaine self-administration sessions (SA) were conducted in a distinct context followed by extinction (EXT) training in a different context. On post-cocaine day 8, rats were re-exposed to the cocaine-paired context (COC-CTX) for 15 min to reactivate cocaine-related memories and then received unilateral microinfusions of anisomycin (ANI, 62.5 µg/0.5 µL) into the BLA plus baclofen/muscimol (B/M, 1.0/0.01 mM/0.5 µL) into the contralateral or ipsilateral DH. Control rats received microinfusions of phosphate buffered saline vehicle (VEH, 0.5 µL) into the corresponding brain regions. Groups then remained in their home cages overnight, followed by additional extinction training until they reached the extinction criterion (≤25 active lever responses/session on two consecutive days) and a test of drug context-induced cocaine seeking. (B) Mean (±SEM) active lever presses during self-administration (SA; mean of the last three training sessions) and during tests of cocaine-seeking behavior in the extinction context (EXT; the last session preceding the test in the cocaine-paired context) and in the cocaine-paired context (COC-paired). (C) Mean (±SEM) inactive lever presses. (D) The time course of active lever responses (mean ± SEM) during the test in the cocaine-paired context. (E) The time course of inactive lever responses (mean ± SEM). (*) Significant difference relative to responding in the extinction context (ANOVA context simple main effect, P < 0.05). (†) Significant difference relative to ipsilateral ANI + B/M treatment (ANOVA surgery-type simple main effect, P < 0.05). (‡) Significant difference relative to VEH treatment (ANOVA treatment simple main effect, P < 0.05). (#) Significant difference relative to all other time intervals (ANOVA time simple main effects, P < 0.05).