Figure 6.

Effect of increasing concentrations of hydralazine on DEM-induced protein carbonylation and hydrogen peroxide production. Rat brain slices were incubated with 10 mM DEM in the absence or presence of increasing concentrations (0.5–500 µM) of hydralazine. After 2 h, aliquots from the incubation media were removed and used to determine H₂O₂ levels using the FOX assay. Slices were homogenized in PEN buffer and aliquots of the homogenate were used to determine PCOs by OxyBlot. Values are expressed as percentage of control and represent the mean ± SEM of 3–4 experiments. Asterisks denote values that are significantly different (p < 0.05) from DEM-treated slices.