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. 2011 Sep 19;194(6):825–839. doi: 10.1083/jcb.201101148

Figure 1.

Figure 1.

Changes in global transcription profile and expression of the genes encoding chromatin-remodeling factors in the epidermis of p63−/− mice. The skin of E16.5 p63−/− and WT mice was processed for LCM to isolate fragments of the epidermis. RNA was isolated from captured tissue, amplified, and processed for microarray and RT-PCR analyses. (A) Cryosection of the skin after LCM including a fragment of the captured epidermis. Bar, 25 µm. (B) Agilent microarray analysis of the laser-captured epidermis of p63−/− and WT mice. Diagrams showing the ontology of the down- and up-regulated genes in p63-null versus WT epidermis. Selected genes involved in the control of nuclear structure and chromatin remodeling, the expression of which was changed in the epidermis of p63−/− versus WT mice, are listed (a full list of the genes is shown in Tables S1 and S2). (C) Real-time PCR for Satb1, Mi-2α, Mi-2β, Terf2, and Prmt4 in the E16.5 epidermis of p63−/− mice normalized to the corresponding levels in age-matched WT mice. Error bars represent SEM.