Table I.

Characteristics of Arf6 mutant proteins

Arf6 mutant protein	Phenotype	Citation	VSVG sortinga
Arf6Q67L	Locked in GTP form	Donaldson, 2003	Apical
Arf6D125N	Binds xanthine nucleotides, presumed nucleotide-free in cells	Yang and Mueckler, 1999	Apical
Arf6T27N	Locked in GDP form	Macia et al., 2004	Basolateral
Arf6N48I	Deficient in PLD activation	Vitale et al., 2005; Jovanovic et al., 2006	Basolateral
Arf6Q67L/Q37E/S38I	Defective in actin remodeling	Al-Awar et al., 2000; Palacios et al., 2001	Apical
Arf6G2A	Can no longer be myristoylated	Knorr et al., 2000	ND
Arf6D125N/G2A	Presumed nucleotide-free, not myristoylated	This paper	Apical
Arf6Q67L/G2A	Locked in GTP form, not myristoylated	This paper	Apical

^aThe VSVG sorting phenotypes in the presence of Arf6 mutant proteins were all obtained in this study.