Table II.

Antibodies used in this study

Antibody	Species	Catalog number	Company	Dilution
α-xM18BP1	Rabbit		A.F. Straight	1.5 µg/ml (IF); 5 µg/ml O/N (WB)
α-xHJURP	Rabbit		A.F. Straight	2 µg/ml (WB)
α-myc	Mouse		A.F. Straight	1 µg/ml (WB)
α–xCENP-A	Rabbit		A.F. Straight	2 µg/ml (WB); 1 µg/ml (IF)
594-α–xCENP-A	Rabbit		A.F. Straight	2 µg/ml (IF)
488-α–xCENP-A	Rabbit		A.F. Straight	2 µg/ml (IF)
α-xAnillin	Rabbit		A.F. Straight	1 µg/ml (WB)
α–xCENP-C	Rabbit		A.F. Straight	1 µg/ml (IF); 1.5 µg/ml (WB)
α–phospho-wee1			J.E. Ferrell	1:1,000 O/N (WB)
α-tubulin	Mouse	T9026	Sigma-Aldrich	0.25 µg/ml (WB)
α-BrdU	Mouse	555627	BD	10 µg/ml (IF)
α–histone H4	Rabbit	ab7311	Abcam	2 µg/ml (WB)
α–histone H2A	Rabbit	ab13923	Abcam	2 µg/ml (WB)
α-FLAG	Mouse	F1804	Sigma-Aldrich	2 µg/ml (IF); 1 µg/ml (WB)
647-anti-myc	Mouse	2233	Cell Signaling Technology	20 µg/ml (IF)

IF, immunofluorescence; O/N, overnight; WB, Western blot.