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. Author manuscript; available in PMC: 2012 Oct 19.
Published in final edited form as: Bioconjug Chem. 2011 Sep 30;22(10):2136–2142. doi: 10.1021/bc200411n

Figure 1.

Figure 1

Inhibition of DsRed expression by PEGylated antisense agents. HEK 293T cells were transfected with pEGFP-N1, pDsRed-Monomer and antisense agent (25 pmol). A. After a 48 h incubation, the media was removed and the cells were imaged. The DsRed channel is shown above the EGFP channel. B. Cells were subsequently lysed and the fluorescence was measured on a BioTek plate reader. DsRed (557/585 nm) fluorescence was normalized to EGFP (485/507 nm) fluorescence. Error bars represent standard deviatons from three independent experiments. AA: non-modified antisense agent. AA-HP: hairpin antisense agent. AA-5K: 5 kDa PEGylated antisense agent. AA-20 kDa: 20 kDa PEGylated antisense agent. AA-40: 40 kDa PEGylated antisense agent.