Figure 4.
Spatial activation of gene expression using a PhotoPEGylated antisense agent. NIH 3T3 cells were transfected with pEGFP-N1, pDsRed-Monomer, and AA-20K PhotoPEG. Only one half side of the well was irradiated for 2 min (25 W, 365 nm) and the cells were imaged after 48 h. The DsRed channel is shown above the EGFP channel. AA: non-modified antisense agent. AA-HP: hairpin antisense agent. AA-20K PhotoPEG: 20 kDa photocleavable antisense agent.
