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Sequences and melting temperatures of PEGylated DsRed antisense agents. Standard deviations were derived from Tm measurements in triplicate.
| Name | DNA Sequence | Tm / °C |
|---|---|---|
| AA | 5’ AACTCGGTGATGACGTTCTCGGAGGAG-NH2 | 69.2 ± 0.7 |
| AA-HP |
|
61.1 ± 0.8 |
| AA-5K | 5’ AACTCGGTGATGACGTTCTCGGAGGAG-NH-PEG5kDa | 63.4 ± 1.3 |
| AA-20K | 5’ AACTCGGTGATGACGTTCTCGGAGGAG-NH-PEG20kDa | 56.1 ± 1.5 |
| AA-40K | 5’ AACTCGGTGATGACGTTCTCGGAGGAG-NH-PEG40kDa | 50.9 ± 1.9 |
