FIGURE 10.

Activation of ERK1/2 in HT1080 cells. Representative Western blot analyses for phosphorylation of ERK1/2 (pERK) are shown for transfected siRHAMM, siScr cells, or nontransfected cells. A, at basal levels after 48 h of treatment with serum-free medium (M, siSRC, siRHAMM) and LMWHA (50 μg/ml); B, during adhesion onto fibronectin using the same controls and treatments. C and D, densitometry analyses of the bands are shown at basal levels (C) and during adhesion (D), respectively, with the ratio of pERK to total ERK1/2 normalized against actin. The results represent the average of three separate experiments in triplicate. Means ± S.E. are plotted; statistical significance is as follows: ***, p ≤ 0.001; **, p ≤ 0.01; *, p ≤ 0.05 compared with the respective control samples.