FIGURE 3.

Effect of siRHAMM on fibronectin-dependent cell adhesion. 48 h after transfection, HT1080 siRHAMM and scramble (siScr)-transfected cells were harvested and seeded (5000 cells) for 30 min on 96-well plates coated with fibronectin (5 μg/cm²). M stands for cell treated with culture medium. The numbers of attached cells were determined using fluorometric CyQUANT assay kit. The results represent the average of three separate experiments in triplicate. Means ± S.E. are plotted; statistical significance is as follows: ***, p ≤ 0.001; **, p ≤ 0.01; *, p ≤ 0.05 compared with the respective control samples. NS, not significant.