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Temperature dependence of activity (a) and heat-induced unfolding recorded by fluorescence (b). Data are shown for the psychrophilic AHA (○), the multiple mutants Mut5 (●) and Mut5CC (■), and the mesophilic PPA (▴) (data from Refs. 22, 44). Activity toward starch as substrate. All experiments were performed at similar protein concentrations (5–40 μg/ml).
