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Normalized reversible endotherms of AHA and of its mutants recorded by DSC in the presence of a nondetergent sulfobetaine. Thermograms were recorded in 30 mm MOPS, 50 mm NaCl, 1 mm CaCl2, pH 7.2, and 1 m 3-(1-pyridinio)-1-propanesulfonate in the case of Mut5 and Mut5CC. Raw data were base line-subtracted and normalized for protein concentration. Inset, same graph including the irreversible thermogram of PPA (44).
