FIGURE 1.

MondoA broadly regulates hexose-induced TXNIP expression. A, HA1ER cells expressing nonsilencing control or MondoA-specific RNAi were glucose (Gluc)-starved overnight and then treated with the indicated hexoses (20 mm; 3h). Western blot analysis of MondoA, TXNIP, ribosomal S6 protein, phospho-S6, and tubulin was performed following treatment. B, control HA1ER cells were transfected with MondoA-V5 and FLAG-Mlx prior to glucose starvation and hexose treatment. Percentage of cells displaying nuclear immunolocalization of MondoA-V5 protein is shown. Error bars, S.D. C, activities of the wild-type (wt) and ChoRE point mutant (−ChoRE) TXNIP luciferase reporters were measured in HA1ER cells following glucose starvation and hexose treatment.