FIGURE 6.

Mutation of Asn-204 but not Asp-194 inhibits complex formation. PS1^−/−/PS2^−/− MEF (dKO) cells were transiently transfected with PE variants and Myc-tagged Pen2 (mPen2) as indicated. After 24 h of expression, cells were lysed, and lysates were either directly loaded on an SDS gel (top) or subjected to immunoprecipitation using anti-GFP antibodies followed by Western blotting. Membranes were probed as indicated. *, unspecific band. Representative blots of at least three independent experiments are shown. nt, non-transfected Swe cells; mat/im, mature/immature; endo, endogenous.