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. 2011 Sep 12;286(44):38783–38794. doi: 10.1074/jbc.M111.257956

FIGURE 6.

FIGURE 6.

Dysfunctional SUV3 impairs mtDNA replication in part because SUV3 associates with replication origin 5. A, representative images of cells expressing MGM101-GFP after depleting SUV3 for various times. Scale bar, 2 μm. B, enumeration of replication foci in MGM101-YS16 strain. C, enumeration of replication foci in MGM101-KYS16 strain. Later time points were not included in B and C because the numbers of cells with replication foci were too low to be compared with early time points. D, enumeration of replication foci for cells expressing MTS, wild-type SUV3, K245A, V272L, or ΔCC mutant in MGM101-YSTAP (D) or MGM101-KYSTAP (E) strains after 8 h of SUV3 depletion in glucose medium (images of cells were collected from two independent experiments; mean ± S.E.; n, number of cells counted; p value calculated using Student's t test, p < 0.001). F, mtIP followed by qPCR showing the selective association of wild-type SUV3 with an active mtDNA replication origin 5 while DSS1 does not bind DNA. mtIP showing association of wild type, K245A, V272L, and ΔCC with replication ori5 in YSTAP (G) and KYSTAP (H) strains. All qPCRs were done in triplicate for statistics. Error bars, S.E.