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. 2011 Sep 11;286(44):38190–38201. doi: 10.1074/jbc.M111.301234

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — ZNF217-F67 binds weakly to the human E-cadherin promoter. A, various GST-ZNF217-F67 constructs were used in EMSA to assess binding to the −230 to −159 region of the human E-cadherin promoter containing the consensus sequence CAGAAC. 1 μg of recombinant protein was used in each lane, and αGST antibody was used to supershift any complexes containing GST fusions. Lanes are: probe only (lane 1), GST only (lanes 2 and 3), human ZNF217-F67 (lanes 4 and 5), human ZNF217-F67 containing the C473A mutation of F6 (lanes 6 and 7), murine ZNF217-F67 (lanes 8 and 9). B, 2 μg of recombinant GST (lane 1), GST-hZNF217-F67 (lane 2), GST-hZNF217-F67 C473A (lane 3), and GST-mZNF217-F67 (lane 4) were used in EMSA with a probe based on a previously identified CAST sequence, containing the consensus sequence CAGAAT (29).