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. 2011 Sep 11;286(44):38190–38201. doi: 10.1074/jbc.M111.301234

FIGURE 3.

FIGURE 3.

ZNF217-F67 binds to a consensus sequence of (T/A)(G/A)CAGAA(T/G/C). A, 2 μg of recombinant GST, GST-ZNF217-F67, and GST-ZNF217-F67 C473A were tested in EMSA for their ability to bind to probes containing either mutations or deletions in and around the core binding consensus of TGCAGAAT. Mutated bases were altered to G residues. The sequences of the probes and the relative strength of binding are given in Table 1. B, 2 μg of recombinant GST, GST-ZNF217-F67, and GST-ZNF217-F67 C473A were tested in EMSA for their ability to bind to probes containing individual site-specific mutations of the TGCAGAAT core DNA binding sequence. The sequences of the probes and the relative strengths of binding are given in Table 2.