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. 2011 Sep 11;286(44):38190–38201. doi: 10.1074/jbc.M111.301234

FIGURE 4.

FIGURE 4.

ZNF217 represses gene expression by sequence specific binding to the TGCAGAAT consensus. A, HEK-293 cells were transfected with 0.25–2 μg of pMT3-FLAG-ZNF217 or pMT3-FLAG-ZNF217 C473A as well as 3 μg of pGL2-(TGCAGAAT)3-LexA-Luc reporter plasmid and 1 μg of LexA-VP16 expression plasmid. Shown are -fold repressions of LexA-VP16 transactivation by ZNF217 or ZNF217 C473A compared with empty vector control (n = 3, error bars show S.D.). B, HEK-293 cells were transfected with 0.25–2 μg of pMT3-FLAG-ZNF217or pMT3-FLAG-ZNF217 C473A as well as 3 μg of either pGL2-(TGCAGAAT)3-LexA-Luc, pGL2-(CAGAAT)3-LexA-Luc, or pGL2-(CTGGAGTA)3-LexA-Luc reporter plasmids and 1 μg of LexA-VP16 expression plasmid. Shown are -fold repressions of LexA-VP16 transactivation by ZNF217 compared with empty vector control (n = 3, error bars show S.D.).