Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Sep 7;286(44):38627–38637. doi: 10.1074/jbc.M111.266809

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 8. — A, naive CD4⁺ T cells stained for pζ-chain (green) and pZAP-70 (red), following treatment with nonlytic C5b-9 and SLE-ICs. Nuclei were stained with DAPI. Shown are the pζ-chain (left), pZAP-70 (2nd left), and merge of pζ-chain and pZAP-70 (2nd right). Both proteins are present in peripheral microclusters and co-localize by 1 h of treatment. IC-treated cells do not show pζ-chain and pZAP-70 (right panel) (enhanced using Photoshop to observe microclusters of pζ and/or pZAP-70). Confocal image at ×630. Figure represents one of five experiments. B, naive CD4⁺ T cells stained for pLAT (red) and pζ-chain (green), treated with SLE-ICs and nonlytic C5b-9. Both proteins are present in microclusters and co-localize after 1 h of treatment (upper panels). Merge of pLAT and pζ-chain on DIC image (upper far right panel) and nuclei stained with DAPI (lower far left panel). Nonlytic C5b-9 treated (lower central panel) and SLE-ICs treated (far right panel). Nonlytic C5b-9 or ICs alone did not phosphorylate either LAT or ζ-chain. Confocal image is at ×630. Figure represents one of five experiments.