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DNA melting measured by 2-AP fluorescence. The 2-AP base (black star in the cartoon) was placed at -4NT in the −12/+8-mt20 (150 nm, filled triangles) and in the -2C:G -mt20 (150 nm, blank triangles). The ratio of 2-AP fluorescence intensity (F) at the individual Rpo41-Mtf1 concentrations to that of the free 2-AP DNA fragments (F0) is representative of DNA melting.
