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Inhibitory effects of ZnCl2 on activity of native and variant enzymes. Shown is the relative hydrogen evolution activity after the addition of 0.05, 0.10, 0.25, or 0.50 mm ZnCl2. Residual activity is relative to enzyme activity with 0 mm ZnCl2 (1.0). Error bars denote S.D. (n = 3–8).