TABLE 2.

Charge-switch mutations altered burst open probability and amplitude of single channel current amplitudes

Single channel recordings were gathered from outside-out patches held at −80 mV in extracellular saline containing 3 mm GABA; means are depicted ±95% confidence interval of fit. Some values were not determined (ND) for both mutants.

Measurement	WT	K328E	K378E
Unitary current (pA)	−2.03 ± 0.007	−1.97 ± 0.029	−1.37 ± 0.057a
Burst Popen	0.79 ± 0.017	0.86 ± 0.003	0.75 ± 0.006
Chord conductance (picosiemens)	26.1 ± 5.09	ND	17.2 ± 5.82
Reversal Potential (mV)	−1.20 ± 10.2	ND	−7.45 ± 19.4

^a Mutants were compared to WT by the Student's t test with significance held at p < 0.05, n = 3.