FIGURE 5.
Functional properties of hα6(N143D+M145V)hβ4*-nAChR. A, representative traces are shown for inward current responses in oocytes held at −70 mV, responding to application of nicotine at the indicated concentrations (shown with the duration of drug exposure as black bars above the traces), and expressing nAChR hα6(N143D+M145V), hβ4, and (hα5/hβ3V9′S) subunits. Calibration bars are 40 nA current (vertical) or 10 s (horizontal). B, results for these and other studies averaged across experiments were used to produce concentration-response curves (ordinate, mean normalized current ± S.E.; abscissa, ligand concentration in log μm) for inward current responses to nicotine, where current amplitudes are represented as a fraction of the peak inward current amplitude in response to the most efficacious concentration of nicotine. See Table 2 for parameters.