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. 2011 Sep 14;286(44):38478–38487. doi: 10.1074/jbc.M111.265710

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — In vivo interaction of FBP21-tandem-WW domains with splicing factors. A, colocalization of SmB-YFP and FBP21-tandem-WW-YFP (upper panel) and SF3B4-CFP and FBP21-tandem-WW-YFP (lower panel) in nuclear speckles. HEK293FT cells were transfected with the respective constructs and analyzed on a Leica SP2 confocal microscope. The bar is 20 μm. B, FRET efficiencies for the energy transfer from SF3B4-CFP and SmB-CFP to FBP21 tandem-WW-YFP were determined as described under “Experimental Procedures.”