TABLE 2.

Thermodynamic constants determined by ITC

S.D. results from at least two experiments. The peptides used in this experiment are listed under supplemental Table S1.

	N	KD	ΔH	−TΔS
		μm	kJ/mol
Smb-1
WT	2.1 ± 0.1	295 ± 70	−21 ± 1	2 ± 1
Smb-2
WT	1.04 ± 0.02	20 ± 1	−37 ± 2	10 ± 2
W29A	1.15 ± 0.20	62 ± 10	−38 ± 3	16 ± 3
W70A	0.98 ± 0.06	60 ± 1	−35 ± 1	12 ± 1
Δ35–41	1.05 ± 0.02	26 ± 4	−37 ± 2	13 ± 2
Δ33–44	0.98 ± 0.02	33 ± 12	−21 ± 5	−3 ± 6
Glycine linker	0.99 ± 0.09	40 ± 9	−46 ± 5	23 ± 6
Smb-4
WT	1.03 ± 0.02	4.5 ± 0.2	−65 ± 4	37 ± 5
W29A	0.74 ± 0.02	50 ± 5	−51 ± 1	28 ± 1
W70A	0.72 ± 0.03	35 ± 2	−55 ± 4	31 ± 4
Δ35–41	1.02 ± 0.01	2.7 ± 0.1	−61 ± 1	32 ± 1
Δ33–44	1.04 ± 0.02	1.8 ± 0. 3	−53 ± 2	22 ± 2
Glycine linker	1.05 ± 0.02	4.7 ± 0. 2	−62 ± 1	33 ± 1
SF3B4–1
WT	2.0 ± 0.1	570 ± 5	−8 ± 1	−9 ± 1
SF3B4–2
WT	1.02 ± 0.02	38 ± 2	−30 ± 1	6 ± 1
W29A	1.04 ± 0.03	134 ± 32	−27 ± 1	7 ± 1
W70A	1.12 ± 0.10	91 ± 11	−19 ± 1	−3 ± 1
Δ35–41	1.03 ± 0.01	38 ± 4	−34 ± 2	10 ± 2
Δ33–44	NDa	ND	ND	ND
Glycine linker	1.11 ± 0.07	43 ± 5	−35 ± 8	12 ± 8

^a ND, not determined.