TABLE 2.
Protein derivatives used in this work
Primers used for construction are listed in supplemental Table S2.
| Construct | Modification | Purpose |
|---|---|---|
| TolCsol_iGS | Membrane barrel replaced (2 × 4GS instead) | SPR, ITC |
| TolCsol_i8H | Membrane barrel replaced (2 × 8H instead) | SPR |
| TolC_c6H | C-terminal 6H | Cross-link bait |
| DevBsol | Membrane anchor replaced (GS instead) | SPR, ITC |
| DevBsol_c8H | Membrane anchor replaced (GS instead), C-terminal 8H | SPR |
| DevBsol_i8H | Membrane anchor replaced (GS instead), hairpin 8H | SPR |
| DevBsol_V469C | Membrane anchor replaced (GS instead), V469C | SPR |
| DevBsol_N333A | Membrane anchor replaced (GS instead), N333A | SPR |
| DevB_N333A | N333A | Complementation |
| DevAC_iGS | Stop codon of DevA removed, 4GS between DevA and DevC | SPR, ITC |
| DevAC_i8H | Stop codon of DevA removed, 8H between DevA and DevC | SPR |
| DevA_c6H | C-terminal 6H | Cross-link bait |