FIGURE 2.

MGAT5-modified N-glycans are essential for eosinophil trafficking and survival. Rolling (A) and adhesion (B) of WT and Mgat5^−/− eosinophils on immobilized VCAM-1 or galectin-3 (Gal-3) under conditions of flow. Rolling on PBS was considered as background. C, chemotaxis of WT and Mgat5^−/− eosinophils in response to eotaxin (100 nm) or medium alone (control) in vitro. Combined data of two independent experiments performed in triplicate for each condition with eosinophils from two different mice for each group is shown for A–C. D, basal [Ca²⁺]_i levels in WT and Mgat5^−/− eosinophils by digital videofluorescence imaging using the Ca2⁺ indicator dye Fura-2 AM. Combined data of three pooled experiments with Mgat5^−/− and WT eosinophils (n = 270–340 cells) from three different mice. E, eosinophil survival evaluated by flow cytometry based on FITC-annexin V binding by gated eosinophils. Combined data of three experiments with eosinophils from three different mice for each group is shown. Data represent mean ± S.E. in A–E. *, p < 0.05 for comparison of WT versus Mgat5^−/− eosinophils.