FIGURE 4.

The p97 N-terminal domain represents a conserved VIM-interacting domain. A, top, sequence alignment of the gp78, ANKZF1, SVIP, and UBXD1 (identified in the accompanying paper by Stapf et al. (46)) VIM. Residues involved in p97N-gp78 interaction are labeled with stars, and residues of the VIM signature motif RX₅AAX₂R are highlighted with red backgrounds. Bottom, streptavidin pull-down assays involving biotinylated VIM peptides (gp78, ANKZF1, and UBXD1) and GST pull-down assay involving GST-tagged SVIP (aa 15–39) and p97ND1 WT and variants. B, surface representation of the p97 VIM interaction site. Residues colored in red represent the core region of the binding domain, which is essential for the respective VIM interaction, and residues colored in orange and yellow contribute moderately to the interaction, whereas residues colored in green are not involved in the respective interaction.