Skip to main content
. 2011 Sep 13;286(44):38679–38690. doi: 10.1074/jbc.M111.274506

FIGURE 6.

FIGURE 6.

Functional studies of the p97-UBXD1 interaction. A, top, domain architecture of UBXD1 and p97. The interactions of the UBXD1 VIM with the N domain and the UBXD1 PUB domain with the C terminus of p97 are indicated. The non-functional UBX domain is colored in black. Bottom, ITC studies of full-length p97 with WT UBXD1, a UBXD1 VIM knock-out (58AAX2R62 58ALX2A62), and a UBXD1 PUB knock-out (K198E/N201E). B, analytical size exclusion chromatography of p97-UBXD1. p97 was incubated with UBXD1 in a 1:0.7 molar ratio. The indicated elution fractions were analyzed by SDS-PAGE followed by Coomassie Brilliant Blue staining.