FIGURE 4.

CBFβ plays an important role in myeloid cell differentiation, especially to the granulocytic lineage. A, qRT-PCR and Western blot analyses of the effects of different siRNAs against CBFβ. NB4 cells were transfected with water, siRNA-NC (siRNA-scramble), or three siRNA strands targeting CBFβ. B and C, morphological analysis of NB4 and HL60 cells at day 3 after electroporation with H₂O, si-NC (siRNA negative control), or si-CBFβ following treatment with 1 μm ATRA. Transient transfection of the cells with si-CBFβ blocked the granulocytic differentiation induced by ATRA. A representative experiment is shown. D, morphological analysis of miR-125b overexpressed NB4 cells at day 3 of treatment with 1 μm ATRA. E, aberrant expression of CBFβ and miR-125b in 10 AML-M3 primary samples, 3 healthy donors were used as controls.