FIGURE 1.

Temperature-dependent reversible aggregation of Cu²⁺-bound PrP. A, reversible aggregation of PrP(23–231) in the presence of Cu²⁺. Gray lines indicate temperature of the sample, and slopes of lines indicate decrease or increase in the temperature. B, reversible aggregation of similar concentrations of PrP(23–231) (circles), Cu²⁺-bound PrP(23–231) (triangles), PrP(90–231) (diamonds), and Cu²⁺-bound PrP(90–231) (squares). The circles, diamonds, and squares are overlapping. Arrows show the directions of experiments. Arrow pointing downward shows cooling, and arrow pointing upward shows heating of sample.