TABLE 2.
Mutant enzymes | Specific activitya | Percentage of specific activity |
---|---|---|
milliunits mg−1 | % | |
Wild type | 2100 | 100 |
E322A | 32 | 1.5 |
E338A | 340 | 16 |
E366A | 0.028 | 0.0013 |
a Enzymatic activities were determined using the cis isomer of Ara2-Hyp-DNS.