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Kinetic properties of recombinant GCP3-S509N mutant. Substrate saturation curve for BCG (A) and NAAG (B), assayed in the presence of either 5 mm Ca2+ or 1 mm Mn2+. Metal saturation curve for Ca2+ (C) and Mn2+(D) assayed in the presence of 5 μm substrate. Error bars, S.D. of two independent experiments.
