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Competitive binding of [OG]EGR-FXa and FFR-NotD to FVa. Observed anisotropy (robs) of 1 nm [OG]EGR-FXa in the absence (●) or presence of 1 (○) or 4 nm (▴) FFR-NotD and 50 μm PC/PS SUVs as a function of total FVa concentration ([FVa]o). Solid lines represent the simultaneous fit by the cubic binding equation with the parameters given in Table 1. Anisotropy titrations were performed and analyzed as described under “Experimental Procedures.”
