FIGURE 4.

HDAC4 regulates HIF-1 transcriptional activity via HIF1α N-terminal lysines. A, HDAC4, HIF1α-WT, HIF1α-mut, or empty vector (Ev) was overexpressed in Hek293 cells as a single agent or in combination along with HRE-firefly and constitutive renilla luciferase. B, HIF1α-WT, HIF1α-mut, or Ev were overexpressed in Hek293 sh-Scr and sh-HDAC4 cells along with HRE-firefly and constitutive renilla luciferase plasmids. 48 h later, dual-luciferase activities were measured and expressed as values relative to the Ev. * and **, p < 0.01. All bars represent mean ± S.D. of three experiments.