TABLE 3.

Kinetic parameters of ApHMWC wild type and mutants

These are apparent values, determined by varying the concentration of one substrate (protein acceptor) at a fixed concentration of the second (sugar donor substrate).

Proteins	Kmapp for HMW1ct	Vmax	kcat	kcat/Km
	μm	nmol min−1mg−1	s−1	m−1s−1
ApHMW1C	5 ± 0.2	415 ± 6	0.489 ∼ 0.504	(9.79∼10.1) × 104
ApHMW1C-F39A	5 ± 1	222 ± 23	0.238 ∼ 0.293	(4.76∼5.86) × 104
ApHMW1C-H214A	6 ± 1	419 ± 29	0.467 ∼ 0.536	(7.78∼8.94) × 104
ApHMW1C-D215A	ND	ND	ND	ND
ApHMW1C-H219A	3 ± 0.5	151 ± 6	0.174 ∼ 0.188	(5.78∼6.26) × 104
ApHMW1C-H272A	6 ± 0.7	297 ± 14	0.339 ∼ 0.372	(5.64∼6.20) × 104