FIGURE 5.

Depletion of CDC50A does not affect Golgi localization of ATP9B but abolishes endosomal localization of ATP11B. HeLa cells stably expressing HA-tagged ATP9B (A, left panels, and B) or ATP11B (A, right panels, and C) were treated with a pool of siRNA for LacZ, CDC50A, CDC50B, or both CDC50A and CDC50B. After 72 h, cells were lysed to isolate total RNA, which was processed for RT-PCR using primers shown in supplemental Table S1 (A). Alternately, cells were fixed, permeabilized, and immunostained for HA and either TGN46 (B), TfnR (C, upper four rows), or calnexin (C, bottom). Bar, 20 μm.